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IRDye? 800CW Goat Anti-Rabbit IgG H&L
Cat. Number:
bs-40295G-IRDye8
Quantity size:
100ul
Concentration:
2mg/ml
Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Background:
IRDye800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
Producer: Li-Cor(USA)
Product Number: 926-32211
Fluorophore: IRDye 800CW(MW: 1166)
Excitation Wavelength: 778nm(in PBS)
Emission Wacelegth: 795nm(in PBS)
Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgG
IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications.
Producer: Li-Cor(USA)
Product Number: 926-32211
Fluorophore: IRDye 800CW(MW: 1166)
Excitation Wavelength: 778nm(in PBS)
Emission Wacelegth: 795nm(in PBS)
Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgG
IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications.
Also known as:
IRDye? 800CW Goat anti-Rabbit IgG H&L; Immunoglobulin G;
Specificity:
●
Goat Polyclonal IgG1, affinity purified by Protein G.
●
Reacts with:
Rabbit
●
Immunogen: Native Rabbit IgG.
Storage:
10 mM TBS (pH=7.4) with 1% BSA, 0.01% Sodium Azide and 50% glycerol. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Application:
WB=1:10000-30000
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.