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Rabbit
Anti-Histone H3 (acetyl K27)/Cy5.5
Cat. Number:
bs-7451R-Cy5.5
Quantity size:
100ul
Concentration:
1mg/ml Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Also known as:
acetyl H3; Histone H3(acetyl k27);
H3K27;
H 3;
H3 3 Like Sequence MH921;
H3 3A;
H3;
H3 Histone;
H3 Histone Family Member E Pseudogene;
H3.4;
H3/A;
H3/g;
H31_HUMAN;
H3F3;
H3FA;
H3FT;
H3t;
HIST1H3J;
HIST3H3;
Histone cluster 1, H3a;
Histone H3 3 Pseudogene;
Histone H3.1;
Histone H3.3;
Histone H3/a;
Histone H3/b;
Histone H3/c;
Histone H3/d;
Histone H3/f;
Histone H3/h;
Histone H3/i;
Histone H3/j;
Histone H3/k;
Histone H3/l;
Specificity:
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Rabbit Polyclonal IgG, affinity purified by Protein A.
●
Reacts with:
(predicted: )
●
Immunogen: KLH conjugated Synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K27.
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Predicted Molecular Weight: 15kDa.
Storage:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Application:
Excitation spectrum: 675nm
Emission spectrum: 694nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.