产品编号 | bs-14601R-AP |
英文名称1 | Rabbit Anti-ENTPD4/AP Conjugated antibody |
中文名称 | 碱性磷酸酶(AP)标记的ENTPD4蛋白抗体 |
别 名 | Apyrase lysosomal; Ectonucleoside triphosphate diphosphohydrolase 4; ENTPD 4; Golgi luminal UDPase; Guanosine diphosphatase like protein; KIAA0392; LALP70; ENTP4_HUMAN; LAP70; LYSAL1; Lysosomal apyrase like 1; Lysosomal apyrase like protein of 70 kDa; NTPDase 4; UDPase; Uridine diphosphatase |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, ) |
产品应用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 70kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ENTPD4 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011] Function: ENTPD4 (Ectonucleoside triphosphate diphosphohydrolase 4) preferentially hydrolyzes nucleoside 5'-diphosphates. Nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP. AMP, ADP, ATP and UMP are not substrates. There are two named isoforms. The preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP. Subcellular Location: Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Note: Localizes in the Golgi and autophagic vacuoles/lysosomes. Tissue Specificity: Ubiquitous. Highest expression in testis and lowest in bladder. Similarity: Belongs to the GDA1/CD39 NTPase family. Database links: Entrez Gene: 9583 Human Entrez Gene: 67464 Mouse Omim: 607577 Human SwissProt: Q9Y227 Human SwissProt: Q9DBT4 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |