产品编号 | bs-10404R |
英文名称 | TRPC1 |
中文名称 | 瞬时受体电位通道1抗体 |
别 名 | TRPC1_HUMAN; Short transient receptor potential channel 1; TrpC1; Transient receptor protein 1; TRP-1; TRP 1; TRP 1 protein; TRP1; TRP1 protein; . HTRP 1; HTRP1; MGC133334; MGC133335; Mtrp1; Transient receptor potential cation channel subfamily C member 1; Transient receptor potential channel 1; Trp related protein 1; TRPC1; Trpc1 transient receptor potential cation channel subfamily C member 1. |
Specific References (1) | bs-10404R has been referenced in 1 publications.
[IF=5.656] Guo J et al. Transient Receptor Potential Canonical 5-Scramblase Signaling Complex Mediates Neuronal Phosphatidylserine Externalization and Apoptosis. Cells. 2020 Feb 26;9(3). IP ; human.
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抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human (predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
产品应用 | ICC=1:100-500, Flow-Cyt=3ug/test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 87kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TRPC1: 710-750/793 <Cytoplasmic> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]. Function: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion. Subunit: Interacts with TRPC4AP. Homotetramer and heterotetramer with TRPC4 and/or TRPC5. Interacts with TRPC3, TRPC4 and TRPC5. Interacts with ITPR3. Interacts with MX1 and RNF24. Interacts with FKBP4. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Seems to be ubiquitous. Similarity: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily. Contains 4 ANK repeats. SWISS: P48995 Gene ID: 7220 Database links: Entrez Gene: 7220 Human Entrez Gene: 22063 Mouse Omim: 602343 Human SwissProt: P48995 Human SwissProt: Q61056 Mouse Unigene: 250687 Human Unigene: 149633 Mouse Unigene: 88592 Rat |
产品图片 |
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-TRPC1 antibody (bs-10404R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |