bs-0450R [Primary Antibody]
Rabbit  Anti-Annexin V Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: Annexin V Rabbit pAb

IR: Immunogen Range:

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 308

Swiss Prot: P08758

Source: recombind human Annexin V: 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008].

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Mouse Uterus tissue lysates Lane 4: Mouse Spleen tissue lysates Lane 5: Mouse Stomach tissue lysates Lane 6: Rat Lung tissue lysates Lane 7: Rat Placenta tissue lysates Lane 8: Rat Spleen tissue lysates Lane 9: Human HepG2 cell lysates Lane 10: Human Panc-1 cell lysates Primary: Anti-Annexin V (bs-0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD
Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-Annexin V  (bs-0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD
Sample: PANC-1(Human) Cell Lysate at 30 ug Primary: Anti-Annexin V  (bs-0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD
Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- Annexin V (bs-0450R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human Neurological glioblastoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96 (blue). Primary Antibody:Rabbit Anti-Annexin V antibody(bs-0450R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (bs-0450R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-0450R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
 
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