bs-9764R [Primary Antibody]
Rabbit  Anti-phospho-STMN1 (Ser63) Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-STMN1 (Ser63) Rabbit pAb

IR: Immunogen Range:41-140/149

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3925

Swiss Prot: P16949

Source: KLH conjugated synthesised phosphopeptide derived from human Stathmin 1 around the phosphorylation site of Ser63:41-140/149 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,Flow-Cyt=1μg/Test

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Cow,Chicken)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STMN1 (Ser63)) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STMN1 (Ser63)) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (bs-9764R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Positive control: (mo)Thymocyte (2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: rabbit Anti-phospho-Stathmin 1 (Ser63) (bs-9764R); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
 
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