bs-6934R [Primary Antibody]
Rabbit  Anti-CD81  Polyclonal Antibody
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Host: Rabbit

Target Protein: CD81

IR: Immunogen Range:101-210/236

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 975

Swiss Prot: P60033

Source: KLH conjugated synthetic peptide derived from human TAPA1/CD81:101-210/236 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000

Cross Reactive Species: Human (predicted: Mouse,Rat)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Recombinant human CD81 protein, hFc (HEK293) Primary: Anti-CD81 (bs-6934R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 36 kDa
Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-CD81 antibody (bs-6934R) Dilution: 2ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line):Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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