bs-7786R [Primary Antibody]
Rabbit  Anti-CDCA2 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: CDCA2 Rabbit pAb

IR: Immunogen Range:361-450/1023

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 157313

Swiss Prot: Q69YH5

Source: KLH conjugated synthetic peptide derived from human CDCA2:361-450/1023 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: CDCA2 is a regulator of chromosome structure during mitosis. CDCA2 forms an essential complex with protein phosphatase PP1-gamma subunit 1 (PPP1CC) and is required for the recruitment of PP1 to chromatin. Knockdown of CDCA2 by RNAi causes large-scale cell death by apoptosis, as does overexpression of a dominant-negative mutant of the protein.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse (predicted: Rat,Rabbit,Pig,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- CDCA2 (bs-7786R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD
Sample: Siha(Human) Cell Lysate at 30 ug Primary: Anti- CDCA2 (bs-7786R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD
araformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCA2) Polyclonal Antibody, Unconjugated (bs-7786R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CDCA2 Polyclonal Antibody, Unconjugated(bs-7786R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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