bs-7551R [Primary Antibody]
Rabbit  Anti-TNFAIP3 interacting protein 3 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: TNFAIP3 interacting protein 3 Rabbit pAb

IR: Immunogen Range:51-150/325

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 79931

Swiss Prot: Q96KP6

Source: KLH conjugated synthetic peptide derived from human TNFAIP3 interacting protein 3/ABIN3:51-150/325 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: ABIN-3 is a member of the A20-binding inhibitor of NF-kappaB activation (ABIN) protein family. Similar to the previously characterized human ABINs (ABIN-1 and ABIN-2), ABIN-3 can bind to A20 and inhibit NF-kappaB activation. In contrast, mouse ABIN-3 is incapable of inhibiting NF-kappaB activation by proinflammatory stimuli because the protein lacks a complete ABIN homology domain, which is required for the funcitonal activity of human ABIN-3.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Mouse,Rat (predicted: Human,Sheep,Cow)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Adrenal gland tissue lysates Lane 3: Mouse Kidney tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Spleen tissue lysates Lane 6: Rat Adrenal gland tissue lysates Lane 7: Rat Kidney tissue lysates Primary: Anti-TNFAIP3 interacting protein 3 (bs-7551R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 40 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TNFAIP3 interacting protein 3) Polyclonal Antibody, Unconjugated (bs-7551R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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