bs-0214R [Primary Antibody]
Rabbit  Anti-DFFA Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: DFFA Rabbit pAb

IR: Immunogen Range:2-100/331

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1676

Swiss Prot: O00273

Source: KLH conjugated synthetic peptide derived from human ICAD:2-100/331 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Caspase-activated deoxyribonuclease(CAD) also designated DNA fragmentation factor 45 kDa subunit (DFF-45)and Caspase-activated DNase. CAD is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. ICAD (DNA fragmentation factor alpha subunit) (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) is inhibitor of the caspase-activated DNase (DFF44).

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: liver (mouse) Lysate at 40 ug lung(mouse) Lysate at 40 ug BRL-3A (rat)cell Lysate at 40 ug Primary: Anti- ICAD (bs-0214R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36kD Observed band size: 36 kD
Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-ICAD (bs-0214R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ICAD Polyclonal Antibody, Unconjugated(bs-0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ICAD/DFF-45 Beta Polyclonal Antibody, Unconjugated(bs-0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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