bs-3793R [Primary Antibody]
Rabbit  Anti-CD75 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: CD75 Rabbit pAb

IR: Immunogen Range:301-406/406

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6480

Swiss Prot: P15907

Source: KLH conjugated synthetic peptide derived from human CD75:301-406/406 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000

Cross Reactive Species: Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with CD75 polyclonal antibody, unconjugated (bs-3793R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- CD75 (bs-3793R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD
Sample: MCF-7(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Primary: Anti- CD75 (bs-3793R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD
 
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