Host: Rabbit
Target Protein: GAPDH
IR: Immunogen Range:1-333/333
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2597
Swiss Prot: P04406
Source: Recombinant mouse GAPDH protein:1-333/333
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, thereversible oxidative phosphorylation of glyceraldehyde-3-phosphatein the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Size: 500ul
Concentration: 1mg/ml
Applications: WB=1:5000-40000, IHC-P=1:100-500, ELISA=1:5000-10000
Cross Reactive Species: Hamster,Rat,Mouse,Human
For research use only. Not intended for diagnostic or therapeutic use.