Host: Rabbit

Target Protein: phospho-ERK1/2 (Thr202 + Tyr204)

IR: Immunogen Range:FL(p-T)E(p-Y)VA

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5595

Swiss Prot: P27361

Source: KLH conjugated Synthesised phosphopeptide derived from mouse p44/42 MAPK around the phosphorylation site of Thr202/Tyr204:FL(p-T)E(p-Y)VA 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: p44/42 MAP Kinase(Phospho-Thr202); ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.