bsm-52159R [Primary Antibody]
Rabbit  Anti-Phospho-Glycogen synthase 1 (Ser641) Recombinant Rabbit mAb   Antibody
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Host: Rabbit

Target Protein: Phospho-Glycogen synthase 1 (Ser641) Recombinant Rabbit mAb

IR: Immunogen Range:PA(p-S)VP

Clonality:

Isotype: IgG

Entrez Gene: 2997

Swiss Prot: P13807

Source: KLH conjugated Synthesised phosphopeptide derived from human Glycogen synthase 1 around the phosphorylation site of Ser641:PA(p-S)VP 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Glycogen Synthase (GS) is a key enzyme in the regulation of glycogen metabolism. GS catalyzes the incorporation of UDP-glucose incorporation into glycogen. The activity of glycogen synthase is regulated by hormonal stimuli (insulin, catecholamines and glucagons) and non-hormonal stimuli (blood glucose level and exercise). Two main isoforms of mammalian GS are designated as muscle (glycogen synthase 1) and liver (glycogen synthase 2). Most tissues express glycogen synthase 1, whereas glycogen synthase 2 appears to be tissue-specific. The two isoforms have 70% identical amino acid sequence. Glycogen synthase can be phosphorylated by multiple kinases including glycogen synthase kinase-3 (GSK-3), mitogen-activated protein kinase-related protein kinase (DYRK), and SAPK2b/p38b which leads to its inactivation.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:1000-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
bsm-52159R_BA02268251_20211015 Phospho-Glycogen synthase 1 (Ser641) (85kD) Sample: Lane 1: Human NIH/3T3 cell lysates Lane 2: Rat Skeletal muscle lysates Lane 3: Rat Heart lysates Primary: Anti-Phospho-Glycogen synthase 1 (Ser641) (bs-15418R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kDa Observed band size: 80 kDa
Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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