Host: Mouse
Target Protein: Firefly Luciferase
IR: Immunogen Range:
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: N/A
Swiss Prot: P08659
Source: Luciferase comes from the North American firefly (Firefly):
Purification: affinity purified by Protein G
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Size: 500ul
Concentration: 1mg/ml
Applications: WB=1:500-5000
Cross Reactive Species: Fruit Fly
For research use only. Not intended for diagnostic or therapeutic use.