bsm-33293M [Primary Antibody]
Mouse  Anti-GSK-3 Beta Monoclonal Antibody
www.bioss.com.cn
sales@bioss.com.cn
support@bioss.com.cn
400-901-9800
DATASHEET

Host: Mouse

Target Protein: GSK-3 Beta

IR: Immunogen Range:

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2932

Swiss Prot: P49841

Source: KLH conjugated synthetic peptide derived from human GSK-3 Beta: 

Purification: affinity purified by Protein G

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Size: 50ul

Concentration: 1mg/ml

Applications: WB(1:500-1000)
ELISA(1:1000-5000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:200-500)

Cross Reactive Species: Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample:
A431 Cell (Human) Lysate at 40 ug
A549 Cell (Human) Lysate at 40 ug
DU145 Cell (Human) Lysate at 40 ug
Primary: Anti- GSK-3 Beta (bsm-33293M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Hela ko GSK-3 Beta (Human) Cell Lysate at 30 ug
Primary: Anti-GSK-3 Beta (bsm-33293M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti-GSK-3 Beta (bsm-33293M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33293M 3A6) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33293M 3A6) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
 
PRODUCT SPECIFIC PUBLICATIONS