bs-18172R [Primary Antibody]
Rabbit  Anti-ISX/Intestine specific homeobox Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: ISX/Intestine specific homeobox Rabbit pAb

IR: Immunogen Range:81-180/245

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 91464

Swiss Prot: Q2M1V0

Source: KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox:81-180/245 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the RAXLX homeobox gene family. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse (predicted: Rat,Sheep,Cow,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Lovo (Human) Cell Lysate at 30 ug Lane 2: SW480 (Human) Cell Lysate at 30 ug Primary: Anti-ISX/Intestine specific homeobox (bs-18172R) at 1:500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Sample: small intestine (Mouse) Lysate at 40 ug large intestine (Mouse) Lysate at 40 ug Primary: Anti-ISX/Intestine specific homeobox (bs-18172R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated (bs-18172R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
 
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