Host: Rabbit
Target Protein: Mus81
IR: Immunogen Range:1-100/551
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 80198
Swiss Prot: Q96NY9
Source: KLH conjugated synthetic peptide derived from human Mus81:1-100/551
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Together, DNA repair and checkpoint responses ensure the integrity of the genome. Coordination of cell cycle checkpoints and DNA repair are especially important following genotoxic radiation or chemotherapy, during which unusually high loads of DNA damage are sustained.
MUS81 encodes a helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae (1). Mus81 is important for replicational stress tolerance in both budding and fission yeast.
Specifically, Mus81 associates with Eme1 to form an endonuclease that can process stalled replication forks before they have regressed to form a Holliday junction.
Mus81 associated endonuclease resolves Holliday junctions into linear duplexes by cutting across the junction exclusively on strands of like polarity.
In addition, Mus81 protein abundance increases in cells following exposure to agents that block DNA replication.
Mus81 is involved in the recruitment of Cds1 to aberrant DNA structures where Cds1 modulates the activity of damage tolerance enzymes. The gene encoding human MUS81 maps to chromosome 11q13 and encodes a 551 amino acid protein.
Size: 100ul
Concentration: 1mg/ml
Applications: IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Cross Reactive Species: (predicted: Human,Mouse,Rat,Macaque Monkey)
For research use only. Not intended for diagnostic or therapeutic use.