bs-17589R [Primary Antibody]
Rabbit  Anti-p21-ARC Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: p21-ARC Rabbit pAb

IR: Immunogen Range:2-100/178

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10094

Swiss Prot: O15145

Source: KLH conjugated synthetic peptide derived from human p21-ARC:2-100/178 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Mouse (predicted: Human,Rat,Rabbit,Cow,Chicken,Xenopus laevis)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with p21-ARC polyclonal antibody, unconjugated (bs-17589R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p21-ARC) Polyclonal Antibody, Unconjugated (bs-17589R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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