bs-14551R [Primary Antibody]
Rabbit  Anti-phospho-EIF4B (Ser406) Rabbit pAb  Polyclonal Antibody
www.bioss.com.cn
sales@bioss.com.cn
techsupport@bioss.com.cn
400-901-9800
DATASHEET

Host: Rabbit

Target Protein: phospho-EIF4B (Ser406) Rabbit pAb

IR: Immunogen Range:HP(p-S)WR

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1975

Swiss Prot: P23588

Source: KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406:HP(p-S)WR 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat (predicted: Sheep,Cow,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD
Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
PRODUCT SPECIFIC PUBLICATIONS