bs-15392R [Primary Antibody]
Rabbit  Anti-GPR91 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: GPR91 Rabbit pAb

IR: Immunogen Range:131-334/334

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 56670

Swiss Prot: Q9BXA5

Source: KLH conjugated synthetic peptide derived from human GPR91:131-334/334 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a G-protein-coupled receptor for succinate, an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development, and it has a potential role in renovascular hypertension which has known correlations to renal failure, diabetes and atherosclerosis. [provided by RefSeq, Oct 2009]

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-GPR91(bs-15392R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39kD Observed band size: 39kD
Protein: 1.kidney lyates(mo);2.liver lyates(mo); Primary: Rabbit Anti-GPR91 (bs-15392R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:39 kD Observed band size:49 kD
Western blot analysis of extracts from Liver tissue(mo) using GPR91 Antibody.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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