bs-12212R [Primary Antibody]
Rabbit  Anti-ZNF342 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: ZNF342 Rabbit pAb

IR: Immunogen Range:231-350/475

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 162979

Swiss Prot: Q8WUU4

Source: KLH conjugated synthetic peptide derived from Human ZNF342/ZNF296:231-350/475 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the Krüppel C2H2-type zinc-finger protein family, ZNF342 (zinc finger protein 342), also known as Zinc finger protein 296, is a 475 amino acid nuclear protein that contains six C2H2-type zinc fingers through which it is thought to be involved in DNA-binding and transcriptional regulation.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse (predicted: Rat,Sheep,Cow,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-ZNF342 (bs-12212R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ZNF342 Polyclonal Antibody, Unconjugated(bs-12212R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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