bs-4289R [Primary Antibody]
Rabbit  Anti-5HT3B receptor Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: 5HT3B receptor Rabbit pAb

IR: Immunogen Range:101-200/441

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9177

Swiss Prot: O95264

Source: KLH conjugated synthetic peptide derived from human 5HTR3B/5HT3B receptor:101-200/441 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test

Cross Reactive Species: Human,Rat (predicted: Mouse,Rabbit,Cow)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lovo Cell (Human) Lysate at 40 ug Primary: Anti- 5HT3B receptor (bs-4289R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 55 kD
Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti- 5HT3B receptor (bs-4289R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugated (bs-4289R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-5HT3B receptor Phospho-c-Fos (Thr325)/AF488 Conjugated antibody (bs-4289R-AF488), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
 
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