bs-0976R [Primary Antibody]
Rabbit  Anti-LPLUNC1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: LPLUNC1 Rabbit pAb

IR: Immunogen Range:21-120/484

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 92747

Swiss Prot: Q8TDL5

Source: KLH conjugated synthetic peptide derived from human LPLUNC1:21-120/484 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. The encoded protein is secreted and is a member of the BPI/LBP/PLUNC protein superfamily. This gene is found with other members of the superfamily in a cluster on chromosome 20. [provided by RefSeq]

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Stomach (Mouse) Lysate at 40 ug Primary: Anti- LPLUNC1 (bs-0976R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- LPLUNC1 (bs-0976R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: Lane1: Lung(Rat) Lysate at 30 ug Lane2:Colon carcinoma(Human) Lysate at 30 ug Primary: Anti-LPLUNC1 (bs-0976R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size: 50kD Observed band size: 53kD
Tissue/cell: human Nasal polyps tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LPLUNC1 Polyclonal Antibody, Unconjugated(bs-0976R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
 
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