bs-6061R [Primary Antibody]
Rabbit  Anti-PIGR Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: PIGR Rabbit pAb

IR: Immunogen Range:31-130/764

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5284

Swiss Prot: P01833

Source: KLH conjugated synthetic peptide derived from human PIGR:31-130/764 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human (predicted: Mouse,Rat,Cow,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-PIGR (bs-6061R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 131 kD
Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-PIGR (bs-6061R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 131 kD
Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIGR) Polyclonal Antibody, Unconjugated (bs-6061R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minu
 
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