Host: Rabbit
Target Protein: Myosin light chain (phospho S20)
IR: Immunogen Range:AT(p-S)NV
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10398
Swiss Prot: P24844
Source: KLH conjugated synthesised phosphopeptide derived from human MYL9 around the phosphorylation site of Ser20:AT(p-S)NV
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues.
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Cross Reactive Species: Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog)
For research use only. Not intended for diagnostic or therapeutic use.