bs-60027R [Primary Antibody]
Rabbit  Anti-Acetyl-Histone H3 (Lys14) Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: Acetyl-Histone H3 (Lys14) Rabbit pAb

IR: Immunogen Range:

Clonality: Polyclonal

Isotype: IgG

Entrez Gene:

Swiss Prot:

Source: : 

Purification: Antigen affinity purification

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-1:2000

Cross Reactive Species: Human (predicted: Mouse,Rat)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr) Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 15 kDa Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 1: HeLa, 2: NIH-3T3, 3: BRL, 4. Mouse liver, 5: Rat liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa
 
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