bs-25232R [Primary Antibody]
Rabbit  Anti-FCGR1A/CD64  Polyclonal Antibody
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Host: Rabbit

Target Protein: FCGR1A/CD64

IR: Immunogen Range:131-230/404

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14129

Swiss Prot: P26151

Source: KLH conjugated synthetic peptide derived from mouse FCGR1A/CD64 :131-230/404 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000

Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Mouse Lung tissue lysates Primary: Anti-FCGR1A/CD64 (bs-25232R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 62 kDa
Blank control（black line）:Raw264.7. Primary Antibody (green line): Rabbit Anti-CD64 antibody (bs-25232R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat Anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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