bsm-51155M [Primary Antibody]
Mouse  Anti-FASN Monoclonal Antibody
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Host: Mouse

Target Protein: FASN

IR: Immunogen Range:

Clonality: Monoclonal

Isotype: IgM

Entrez Gene: 2194

Swiss Prot: P49327

Source: KLH conjugated synthetic peptide derived from human FASN: 

Purification: affinity purified by Protein AGL

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: FASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:200-1000,IHC-P=1:100-500,IHC-F=1:20-200,ICC=1:100-500,IF=1:20-200

Cross Reactive Species: Human (predicted: Mouse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Primary: Anti-FASN (bsm-51155M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 274 kDa Observed band size: 270 kDa
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
 
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