bsm-33117M [Primary Antibody]
Mouse  Anti-NFKB p65 Monoclonal Antibody
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Host: Mouse

Target Protein: NFKB p65

IR: Immunogen Range:

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 5970

Swiss Prot: Q04206

Source: Recombinant human NFKB p65 Protein: 

Purification: affinity purified by Protein G

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Jurkat Cell (Human) Lysate at 40 ug Primary: Anti- NFKB p65 (bsm-33117M) at 1/2 000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 65 kD
Sample: Hela Cell (Human) Lysate at 40 ug A431 Cell (Human) Lysate at 40 ug K562 Cell (Human) Lysate at 40 ug Primary: Anti- NFKB p65 (bsm-33117M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 65 kD
Sample: RELA-KO(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-NFKB p65 (bsm-33117M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD
Sample: HL60A549(Human) Cell Lysate at 30 ug Primary: Anti-NFKB p65 (bsm-33117M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-MouseIgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 62 kD
Sample: MCF-7(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Raw264.7(Mouse) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-NFKB p65 (bsm-33117M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 62 kD
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (ascites of bsm-33117M 7G6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (ascites of bsm-33117M 7G6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (ascites of bsm-33117M 7G6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (ascites of bsm-33117M 7G6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (ascites of bsm-33117M 7G6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFKB p65) Monoclonal Antibody, Unconjugated (ascites of bsm-33117M 7G6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
 
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