bs-18457R [Primary Antibody]
Rabbit  Anti-ZBTB9/ZNF919  Polyclonal Antibody
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Host: Rabbit

Target Protein: ZBTB9/ZNF919

IR: Immunogen Range:331-430/473

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 221504

Swiss Prot: Q96C00

Source: KLH conjugated synthetic peptide derived from human ZBTB9/ZNF919:331-430/473 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: ZBTB9 is a 473 amino acid member of the Krüppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZBTB9 contains a BTB domain, also known as a POZ domain, which inhibits DNA binding and mediates homotypic and heterotypic dimerization. Characteristics of the BTB domain suggest that ZBTB43 functions as a transcription regulator.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Du145(Human) Cell Lysate at 40 ug Lovo(Human) Cell Lysate at 40 ug Primary: Anti-ZBTB9/ZNF919 (bs-18457R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (bs-18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (bs-18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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