bs-13239R [Primary Antibody]
Rabbit  Anti-FZD3  Polyclonal Antibody
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Host: Rabbit

Target Protein: FZD3

IR: Immunogen Range:121-220/666

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7976

Swiss Prot: Q9NPG1

Source: KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3:121-220/666 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Protein: pancreas(mouse) lysates at 30ug; Primary: Anti-FZD3 (bs-13239R) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kD
Protein: brain(Rat) lysates at 30ug; Primary: Anti-FZD3 (bs-13239R) at 1:300; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kD, 25kD
Sample: Lane 1: Human SW480 cell Lysates Lane 2: Human U-2 OS cell Lysates Lane 3: Human A431 cell Lysates Lane 4: Human SH-SY5Y cell Lysates Primary: Anti-FZD3 (bs-13239R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74kDa Observed band size: 70kDa
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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