bs-5628R [Primary Antibody]
Rabbit  Anti-phospho-Alpha synuclein (Ser129)  Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-Alpha synuclein (Ser129)

IR: Immunogen Range:MP(p-S)EE

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6622

Swiss Prot: P37840

Source: KLH conjugated Synthesised phosphopeptide derived from human SNCA around the phosphorylation site of Ser129:MP(p-S)EE 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]..

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: RAW264.7 (Mouse) CellLysate at 40 ug Primary: Anti-phospho-Alpha synuclein(Ser129)(bs-5628R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 15 kD
Sample: Lane 1: Human SY5Y cell lysates Lane 2: Human U87MG cell lysates Lane 3: Human U251 cell lysates Lane 4: Human HUVEC Cell Lysates Lane 5: Human HELA Cell Lysates Lane 6: Human 293T Cell Lysates Primary: Anti-phospho-Alpha synuclein (Ser129) (bs-5628R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 18 kDa
Sample: Lane 1: Cerebellum (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Cerebellum (Rat) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 6: U251 (Human) Cell Lysate at 30 ug Lane 7: 293T (Human) Cell Lysate at 30 ug Lane 8: Hela (Human) Cell Lysate at 30 ug Primary: Anti-phospho-Alpha synuclein (Ser129) (bs-5628R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD
Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-phospho-Alpha synuclein(Ser129) antibody (bs-5628R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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