bs-0426R [Primary Antibody]
Rabbit  Anti-MOG Polyclonal Antibody
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Host: Rabbit

Target Protein: MOG

IR: Immunogen Range:35-55/247

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4340

Swiss Prot: Q61885

Source: KLH conjugated synthetic peptide derived from mouse MOG:35-55/247 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Mouse,Rat (predicted: Human,Pig,GuineaPig)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- Myelin-oligodendrocyte glycoprotein (bs-0426R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kD
Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti- Myelin-oligodendrocyte glycoprotein (bs-0426R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kD
Sample: Lane 1: Mouse Spinal cord tissue lysates Lane 2: Rat Spinal cord tissue lysates Primary: Anti-MOG (bs-0426R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 26 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (bs-0426R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (bs-0426R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti- MOG Polyclonal Antibody, Unconjugated(bs-0426R) 1:200, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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