bs-3836R [Primary Antibody]
Rabbit  Anti-PABP (Methyl Arg455/460)  Polyclonal Antibody
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Host: Rabbit

Target Protein: PABP (Methyl Arg455/460)

IR: Immunogen Range:GAI(Me-R)PAAP(Me-R)PP

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 26986

Swiss Prot: P11940

Source: KLH conjugated synthesised metylpeptide derived from human PABP around the metylation site of Arg455/Arg460:GAI(Me-R)PAAP(Me-R)PP 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq, Aug 2010].

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Chicken,GuineaPig,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PABP (Methyl Arg455,460)) Polyclonal Antibody, Unconjugated (bs-3836R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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