bs-2845R [Primary Antibody]
Rabbit  Anti-APG4B(Acetyl K39)  Polyclonal Antibody
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Host: Rabbit

Target Protein: APG4B(Acetyl K39)

IR: Immunogen Range:

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23192

Swiss Prot: Q9Y4P1

Source: KLH conjugated synthetic peptide derived from human APG4B(Acetyl K39): 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: ATG4B is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Size: 200ul

Concentration: 1mg/ml

Applications: IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat (predicted: Pig)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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