bs-2590R [Primary Antibody]
Rabbit  Anti-NRSF Polyclonal Antibody
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Host: Rabbit

Target Protein: NRSF

IR: Immunogen Range:401-450/1097

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5978

Swiss Prot: Q13127

Source: KLH conjugated synthetic peptide derived from human NRSF:401-450/1097 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Hela(Human) Cell Lysate at 30 ug Hela KO NRSF (Human) Cell Lysate at 30 ug Primary: Anti- NRSF (bs-2590R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD
Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (bs-2590R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD
NRSF was immunoprecipitated from human hela cells lysate with bs-2590R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: human hela cells lysate 10 µg (Input). Lane 2: bs-2590R IP in human hela cells lysate. Lane 3: native rabbit IgG IP in human hela cells lysate (negative control). Secondary All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(bs-2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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