bs-11324R [Primary Antibody]
Rabbit  Anti-Nova1 Polyclonal Antibody
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Host: Rabbit

Target Protein: Nova1

IR: Immunogen Range:411-510/510

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4857

Swiss Prot: P51513

Source: KLH conjugated synthetic peptide derived from human Nova1:411-510/510 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Nova-1 and Nova-2 are members of a superfamily of protein regulators of RNA metabolism in neurons. Both are nuclear RNA binding proteins with K homology motifs, conserved protein sequences which bind to RNA (1,2). Nova proteins, normally sequestered in the central nervous system, are expressed by systemic tumors in patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA) (3,4). Nova-1 is expressed in the hindbrain and ventral spinal cord and Nova-2 is expressed in the neocortex and hippocampus (4). Nova-1 is necessary for regulating neuron-specific alternative splicing of the glycine receptor Alpha2 pre-mRNA (5).

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat (predicted: Cow,Chicken)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Nova1 (bs-11324R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Sample: DU145(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- Nova1 (bs-11324R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 50 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Nova1 (bs-11324R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (bs-11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (bs-11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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