bs-1297R [Primary Antibody]
Rabbit  Anti-CD28 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: CD28 Rabbit pAb

IR: Immunogen Range:1-120/218

Clonality: Polyclonal

Isotype:

Entrez Gene: 12487

Swiss Prot: P31041

Source: KLH conjugated synthetic peptide derived from mouse CD28:1-120/218 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Size: 200ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test

Cross Reactive Species: Mouse,Rat (predicted: Human,Pig,Cow,Chicken,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(bs-1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(bs-1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody Antibody: Rabbit IgG; Supplier: Cell Signaling Technology; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody Antibody: Goat anti-rabbit IgG-FITC; Supplier: Cell Signaling Technology; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody Supplier: Bioss; Supplier catalog number: bs-1297R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
 
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