bs-4618R [Primary Antibody]
Rabbit  Anti-ICAM1  Polyclonal Antibody
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Host: Rabbit

Target Protein: ICAM1

IR: Immunogen Range:51-100/532

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3383

Swiss Prot: P05362

Source: KLH conjugated synthetic peptide derived from human CD54/ICAM-1:51-100/532 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: U937 Cell (Human) Lysate at 40 ug Primary: Anti-ICAM1 (bs-4618R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 60 kD
Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti- ICAM1 (bs-4618R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD
Sample: Raji Cell (Human) Lysate at 40 ug Primary: Anti-ICAM1 (bs-4618R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1) Polyclonal Antibody, Unconjugated (bs-4618R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): U937 (fixed with 2% paraformaldehyde for 10 min at room temperature). Primary Antibody (green line): Rabbit Anti-ICAM1 antibody (bs-4618R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
 
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