bs-4212R [Primary Antibody]
Rabbit  Anti-Neuroligin 1 Polyclonal Antibody
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Host: Rabbit

Target Protein: Neuroligin 1

IR: Immunogen Range:701-800/863

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 22871

Swiss Prot: Q8N2Q7

Source: KLH conjugated synthetic peptide derived from human Neuroligin 1:701-800/863 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Neuroligin 1 is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors, channels, and signal-transduction molecules to synaptic sites of cell adhesion. In addition, the neuroligin/beta-neurexin junction may be involved in the excitatory/inhibitory specification of CNS neuron synapses. A role for Neuroligin 1 is also suggested in autism.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Neuroligin 1 (bs-4212R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kD
Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-Neuroligin 1 (bs-4212R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Neuroligin 1 (bs-4212R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kD
Blank control (blue line): U251 (fixed with 70% ethanol for 5 min at -20℃). Primary Antibody (green line): Rabbit Anti- Neuroligin 1 antibody (bs-4212R), Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
Blank control(blue): Mouse brain cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti- Neuroligin 1 /PE Conjugated antibody (bs-4212R /PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.
 
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