bsm-60286R [Primary Antibody]
Rabbit  Anti-LYPD3  Antibody
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Host: Rabbit

Target Protein: LYPD3

IR: Immunogen Range:

Clonality:

Isotype: IgG

Entrez Gene: 27076

Swiss Prot: O95274

Source: KLH conjugated synthetic peptide derived from human LYPD3: 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Predicted to enable laminin binding activity. Predicted to act upstream of or within cell-matrix adhesion. Located in extracellular space. [provided by Alliance of Genome Resources, Nov 2021]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-1000,IHC-P=1:100-500,ICC=1:20-50,IF=1:20-100

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: A431 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 36 kDa Observed MW: 70 kDa
Tissue: Human skin Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60286R
Tissue: Human esophagus Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60286R
Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60286R
Cell line: A431 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60286R
 
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