bsm-51566M [Primary Antibody]
Mouse  Anti-YY1  Monoclonal Antibody
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Host: Mouse

Target Protein: YY1

IR: Immunogen Range:

Clonality: Monoclonal

Isotype: IgG2a

Entrez Gene: 7528

Swiss Prot: P25490

Source: Recombinant human YY1 (His-tagged).: 

Purification: affinity purified by Protein G

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: YY1 is a ubiquitously distributed transcription factor belonging to the GLI Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-1000,IHC-P=1:25-50,IHC-F=1:25-50,IF=1:100-500

Cross Reactive Species: Human,Rat,Zebrafish,Fish,Monkey

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: 293 cell lysates Lane 2: C6 cell lysates Lane 3: Hela cell lysates Lane 4: U-251MG cell lysates Primary: Anti-YY1 (bsm-51566M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 65 kD
Sample: Lane 1: Daudi cell lysates Lane 2: U251 cell lysates Primary: Anti-YY1 (bsm-51566M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 68 kD
Paraformaldehyde-fixed, paraffin embedded (Monkey brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YY1(Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YY1(Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Zebra fish brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YY1(Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
 
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