bsm-52938R [Primary Antibody]
Rabbit  Anti-TNFR2 Recombinant Rabbit mAb   Antibody
www.bioss.com.cn
sales@bioss.com.cn
techsupport@bioss.com.cn
400-901-9800
DATASHEET

Host: Rabbit

Target Protein: TNFR2 Recombinant Rabbit mAb

IR: Immunogen Range:415-461/461

Clonality:

Isotype: IgG

Entrez Gene: 7133

Swiss Prot: P20333

Source: A synthesized peptide derived from human TNFRSF1B:415-461/461 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200

Cross Reactive Species: Human (predicted: Mouse,Rat)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Human THP-1 cell Lysates Lane 2: Human K562 cell Lysates Lane 3: Human U937 cell Lysates Lane 4: Human U87MG cell Lysates Lane 5: Human HUVEC cell Lysates Primary: Anti-TNFR2 (bsm-52938R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46kDa Observed band size: 70 kDa
Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (bsm-52938R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (bsm-52938R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (bsm-52938R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
 
PRODUCT SPECIFIC PUBLICATIONS