VALIDATION IMAGES
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- AKT1 (bsm-52010R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Thymus (Mouse) Lysate at 40 ug
Lane 3: Heart (Mouse) Lysate at 40 ug
Lane 4: Lung (Mouse) Lysate at 40 ug
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 6: Cerebellum (Rat) Lysate at 40 ug
Lane 7: Thymus (Rat) Lysate at 40 ug
Lane 8: Heart (Rat) Lysate at 40 ug
Lane 9: MCF-7 (Human) Cell Lysate at 30 ug
Lane 10: Hela (Human) Cell Lysate at 30 ug
Lane 11: U937 (Human) Cell Lysate at 30 ug
Lane 12: A549 (Human) Cell Lysate at 30 ug
Primary: Anti- AKT1 (bsm-52010R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.