bs-33016R [Primary Antibody]
Rabbit  Anti-Strep-Tag II Polyclonal Antibody
www.bioss.com.cn
sales@bioss.com.cn
techsupport@bioss.com.cn
400-901-9800
DATASHEET

Host: Rabbit

Target Protein: Strep-Tag II

IR: Immunogen Range:

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: N/A

Swiss Prot: N/A

Source: KLH conjugated Strep-Tag: 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000

Cross Reactive Species: Species independent

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) Lane 2: Negative control Primary: Anti-Strep-Tag II (bs-33016R) at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Sample: Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug Lane 2: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug Primary: Anti-Strep-Tag II (bs-33016R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
 
PRODUCT SPECIFIC PUBLICATIONS