bsm-33041M [Primary Antibody]
Mouse  Anti-beta I Tubulin (Loading Control) Monoclonal Antibody
www.bioss.com.cn
sales@bioss.com.cn
techsupport@bioss.com.cn
400-901-9800
DATASHEET

Host: Mouse

Target Protein: beta I Tubulin (Loading Control)

IR: Immunogen Range:

Clonality: Monoclonal

Isotype: IgG2a

Entrez Gene: 81027

Swiss Prot: Q9H4B7

Source: KLH conjugated synthetic peptide derived from human beta I Tubulin: 

Purification: affinity purified by Protein G

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:5000-20000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1:100-500,ICC=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: K562 Cell (Human) Lysate at 40 ug Jurkat Cell (Human) Lysate at 40 ug Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: 293T(Human) Cell Lysate at 30 ug Cerebellum (Mouse) Lysate at 40 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Lane 3: Cerebrum (Mouse) Lysate at 40 ug Lane 4: Fetal brain (Mouse) Lysate at 40 ug Lane 5: Heart (Mouse) Lysate at 40 ug Lane 6: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 7: Spleen (Rat) Lysate at 40 ug Lane 8: Spleen (Mouse) Lysate at 40 ug Lane 9: Ovary (Rat) Lysate at 40 ug Primary: Anti-beta I Tubulin (bsm-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (ascites of bsm-33041M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (ascites of bsm-33041M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M 5F7) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0296G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
 
PRODUCT SPECIFIC PUBLICATIONS