bs-2499R [Primary Antibody]
Rabbit  Anti-CD69  Polyclonal Antibody
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Host: Rabbit

Target Protein: CD69

IR: Immunogen Range:101-199/199

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 969

Swiss Prot: Q07108

Source: KLH conjugated synthetic peptide derived from human CD69:101-199/199 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse (predicted: Rat,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: U937 Cell Lysate at 30 ug Primary: Anti- CD69 (bs-2499R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 34 kD
Sample: Lane 1: Blood cell (Mouse) Lysate at 40 ug Lane 2: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CD69 (bs-2499R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33/27 kD Observed band size: 30 kD
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)) . Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (bs-2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
 
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