bs-21073R [Primary Antibody]
Rabbit  Anti-p66 beta/GATAD2B Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: p66 beta/GATAD2B Rabbit pAb

IR: Immunogen Range:381-480/593

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 57459

Swiss Prot: Q8WXI9

Source: KLH conjugated synthetic peptide derived from human p66 beta:381-480/593 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a zinc finger protein transcriptional repressor. The encoded protein is part of the methyl-CpG-binding protein-1 complex, which represses gene expression by deacetylating methylated nucleosomes. Mutations in this gene are linked to intellectual disability and dysmorphic features associated with mental retardation. [provided by RefSeq, Jun 2016]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-p66 beta (bs-21073R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p66 beta/GATAD2B) Polyclonal Antibody, Unconjugated (bs-21073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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