bs-14024R [Primary Antibody]
Rabbit  Anti-CPNE1 Polyclonal Antibody
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Host: Rabbit

Target Protein: CPNE1

IR: Immunogen Range:1-120/537

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 615677

Swiss Prot: Q99829

Source: KLH conjugated synthetic peptide derived from human CPNE1:1-120/537 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Copine I is a member of the copine family of evolutionarily conserved, soluble, calcium-dependent, membrane-binding proteins. Members of the copine family are involved in signal transduction and membrane trafficking. Copine I is ubiquitously expressed and contains two N-terminal C2 domains and one C-terminal VWFA (von Willebrand factor A) domain, which is also referred to as the A domain or the core domain. As is characteristic of the copine family, copine I functions in membrane trafficking and is capable of binding phospholipids in a calcium-dependent manner. Copine I is also able to bind liposomes. Via its VWFA domain, copine I directly interacts with the ubiquitin-conjugating enzyme, UBC12, and may play a role in the regulation of TNF alpha-signaling.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse (predicted: Rat,Pig,Sheep,Cow)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-CPNE1 (bs-14024R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 59 kD Observed band size: 59 kD
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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